Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy
This review article is aimed at providing an outline of the present information base available on the applications of CRISPR/Cas9 in cancer therapy and the challenges raised by its implementation. We will first explain the mechanism of action and other key components pertaining to the functioning of CRISPR/Cas9 technology. Further, we will provide case studies that have had some success in pre-clinical and clinical applications of CRISPR/Cas9. However, there are several challenges that prevent the wide application of this very promising approach of CRISPR/Cas9 in cancer therapy. Among them are off-target effects and unintended mutations, difficulties in delivery, targeting specificity, etc. Specificity enhancement, improvement in delivery systems, and solutions against regulatory challenges have been described.
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Recombinant DNA Technology, CRISPER/Cas-9, Applications, Challenges, Genome, Genome Engineering.
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(1) Ahmad Jalal Masood
Graduate, Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
(2) Ejaz Ahmed
Graduate, Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
(3) Rimsha Iftikhar
Graduate, Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
(4) Laiba Javed
Graduate, Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
(5) Aimen Sahar
Graduate, Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
(6) Muhammad Hossain
Graduate, Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.
Cite this article
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APA : Masood, A. J., Ahmed, E., & Iftikhar, R. (2024). Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy. Global Pharmaceutical Sciences Review, IX(I), 10-28. https://doi.org/10.31703/gpsr.2024(IX-I).02
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CHICAGO : Masood, Ahmad Jalal, Ejaz Ahmed, and Rimsha Iftikhar. 2024. "Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy." Global Pharmaceutical Sciences Review, IX (I): 10-28 doi: 10.31703/gpsr.2024(IX-I).02
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HARVARD : MASOOD, A. J., AHMED, E. & IFTIKHAR, R. 2024. Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy. Global Pharmaceutical Sciences Review, IX, 10-28.
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MHRA : Masood, Ahmad Jalal, Ejaz Ahmed, and Rimsha Iftikhar. 2024. "Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy." Global Pharmaceutical Sciences Review, IX: 10-28
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MLA : Masood, Ahmad Jalal, Ejaz Ahmed, and Rimsha Iftikhar. "Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy." Global Pharmaceutical Sciences Review, IX.I (2024): 10-28 Print.
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OXFORD : Masood, Ahmad Jalal, Ahmed, Ejaz, and Iftikhar, Rimsha (2024), "Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy", Global Pharmaceutical Sciences Review, IX (I), 10-28
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TURABIAN : Masood, Ahmad Jalal, Ejaz Ahmed, and Rimsha Iftikhar. "Applications and Challenges of CRISPER/Cas-9 System in Cancer Therapy." Global Pharmaceutical Sciences Review IX, no. I (2024): 10-28. https://doi.org/10.31703/gpsr.2024(IX-I).02